RT Journal
A1 Grey, Corinne
A1 Clément, Julie A.J.
A1 Buard, Jérôme
A1 Leblanc, Benjamin
A1 Gut, Ivo
A1 Gut, Marta
A1 Duret, Laurent
A1 de Massy, Bernard
T1 In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites
JF Genome Research 
JO Genome Research 
YR 2017 
FD April 01 
VO 27 
IS 4 
SP 580 
OP 590 
DO 10.1101/gr.217240.116 
UL http://genome.cshlp.org/content/27/4/580.abstract 
AB In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis.