TY  - JOUR
A1  - Grey, Corinne
A1  - Clément, Julie A.J.
A1  - Buard, Jérôme
A1  - Leblanc, Benjamin
A1  - Gut, Ivo
A1  - Gut, Marta
A1  - Duret, Laurent
A1  - de Massy, Bernard
T1  - In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites
Y1  - 2017/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 580 
EP  - 590 
DO  - 10.1101/gr.217240.116 
VL  - 27 
IS  - 4 
UR  - http://genome.cshlp.org/content/27/4/580.abstract 
N2  - In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis. 
ER  -