@article{Grey01042017,
author = {Grey, Corinne and Clément, Julie A.J. and Buard, Jérôme and Leblanc, Benjamin and Gut, Ivo and Gut, Marta and Duret, Laurent and de Massy, Bernard}, 
title = {In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites},
volume = {27}, 
number = {4}, 
pages = {580-590}, 
year = {2017}, 
doi = {10.1101/gr.217240.116}, 
abstract ={In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis.}, 
URL = {http://genome.cshlp.org/content/27/4/580.abstract}, 
eprint = {http://genome.cshlp.org/content/27/4/580.full.pdf+html}, 
journal = {Genome Research} 
}