TY  - JOUR
A1  - Reuß, Daniel R.
A1  - Altenbuchner, Josef
A1  - Mäder, Ulrike
A1  - Rath, Hermann
A1  - Ischebeck, Till
A1  - Sappa, Praveen Kumar
A1  - Thürmer, Andrea
A1  - Guérin, Cyprien
A1  - Nicolas, Pierre
A1  - Steil, Leif
A1  - Zhu, Bingyao
A1  - Feussner, Ivo
A1  - Klumpp, Stefan
A1  - Daniel, Rolf
A1  - Commichau, Fabian M.
A1  - Völker, Uwe
A1  - Stülke, Jörg
T1  - Large-scale reduction of the Bacillus subtilis genome: consequences for the transcriptional network, resource allocation, and metabolism
Y1  - 2017/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 289 
EP  - 299 
DO  - 10.1101/gr.215293.116 
VL  - 27 
IS  - 2 
UR  - http://genome.cshlp.org/content/27/2/289.abstract 
N2  - Understanding cellular life requires a comprehensive knowledge of the essential cellular functions, the components involved, and their interactions. Minimized genomes are an important tool to gain this knowledge. We have constructed strains of the model bacterium, Bacillus subtilis, whose genomes have been reduced by ∼36%. These strains are fully viable, and their growth rates in complex medium are comparable to those of wild type strains. An in-depth multi-omics analysis of the genome reduced strains revealed how the deletions affect the transcription regulatory network of the cell, translation resource allocation, and metabolism. A comparison of gene counts and resource allocation demonstrates drastic differences in the two parameters, with 50% of the genes using as little as 10% of translation capacity, whereas the 6% essential genes require 57% of the translation resources. Taken together, the results are a valuable resource on gene dispensability in B. subtilis, and they suggest the roads to further genome reduction to approach the final aim of a minimal cell in which all functions are understood. 
ER  -