TY  - JOUR
A1  - Wang, Jiaxu
A1  - Jenjaroenpun, Piroon
A1  - Bhinge, Akshay
A1  - Angarica, Vladimir Espinosa
A1  - Del Sol, Antonio
A1  - Nookaew, Intawat
A1  - Kuznetsov, Vladimir A.
A1  - Stanton, Lawrence W.
T1  - Single-cell gene expression analysis reveals regulators of distinct cell subpopulations among developing human neurons
Y1  - 2017/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1783 
EP  - 1794 
DO  - 10.1101/gr.223313.117 
VL  - 27 
IS  - 11 
UR  - http://genome.cshlp.org/content/27/11/1783.abstract 
N2  - The stochastic dynamics and regulatory mechanisms that govern differentiation of individual human neural precursor cells (NPC) into mature neurons are currently not fully understood. Here, we used single-cell RNA-sequencing (scRNA-seq) of developing neurons to dissect/identify NPC subtypes and critical developmental stages of alternative lineage specifications. This study comprises an unsupervised, high-resolution strategy for identifying cell developmental bifurcations, tracking the stochastic transcript kinetics of the subpopulations, elucidating regulatory networks, and finding key regulators. Our data revealed the bifurcation and developmental tracks of the two NPC subpopulations, and we captured an early (24 h) transition phase that leads to alternative neuronal specifications. The consequent up-regulation and down-regulation of stage- and subpopulation-specific gene groups during the course of maturation revealed biological insights with regard to key regulatory transcription factors and lincRNAs that control cellular programs in the identified neuronal subpopulations. 
ER  -