RT Journal
A1 Bulkescher, Ruben
A1 Starkuviene, Vytaute
A1 Erfle, Holger
T1 Solid-phase reverse transfection for intracellular delivery of functionally active proteins
JF Genome Research 
JO Genome Research 
YR 2017 
FD October 01 
VO 27 
IS 10 
SP 1752 
OP 1758 
DO 10.1101/gr.215103.116 
UL http://genome.cshlp.org/content/27/10/1752.abstract 
AB Delivery of large and functionally active biomolecules across cell membranes presents a challenge in cell biological experimentation. For this purpose, we developed a novel solid-phase reverse transfection method that is suitable for the intracellular delivery of proteins into mammalian cells with preservation of their function. We show results for diverse application areas of the method, ranging from antibody-mediated inhibition of protein function to CRISPR/Cas9-based gene editing in living cells. Our method enables prefabrication of “ready to transfect” substrates carrying diverse proteins. This allows their easy distribution and standardization of biological assays across different laboratories.