RT Journal
A1 Wessels, Hans-Hermann
A1 Imami, Koshi
A1 Baltz, Alexander G.
A1 Kolinski, Marcin
A1 Beldovskaya, Anastasia
A1 Selbach, Matthias
A1 Small, Stephen
A1 Ohler, Uwe
A1 Landthaler, Markus
T1 The mRNA-bound proteome of the early fly embryo
JF Genome Research 
JO Genome Research 
YR 2016 
FD July 01 
VO 26 
IS 7 
SP 1000 
OP 1009 
DO 10.1101/gr.200386.115 
UL http://genome.cshlp.org/content/26/7/1000.abstract 
AB Early embryogenesis is characterized by the maternal to zygotic transition (MZT), in which maternally deposited messenger RNAs are degraded while zygotic transcription begins. Before the MZT, post-transcriptional gene regulation by RNA-binding proteins (RBPs) is the dominant force in embryo patterning. We used two mRNA interactome capture methods to identify RBPs bound to polyadenylated transcripts within the first 2 h of Drosophila melanogaster embryogenesis. We identified a high-confidence set of 476 putative RBPs and confirmed RNA-binding activities for most of 24 tested candidates. Most proteins in the interactome are known RBPs or harbor canonical RBP features, but 99 exhibited previously uncharacterized RNA-binding activity. mRNA-bound RBPs and TFs exhibit distinct expression dynamics, in which the newly identified RBPs dominate the first 2 h of embryonic development. Integrating our resource with in situ hybridization data from existing databases showed that mRNAs encoding RBPs are enriched in posterior regions of the early embryo, suggesting their general importance in posterior patterning and germ cell maturation.