TY  - JOUR
A1  - Orioli, Andrea
A1  - Praz, Viviane
A1  - Lhôte, Philippe
A1  - Hernandez, Nouria
T1  - Human MAF1 targets and represses active RNA polymerase III genes by preventing recruitment rather than inducing long-term transcriptional arrest
Y1  - 2016/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 624 
EP  - 635 
DO  - 10.1101/gr.201400.115 
VL  - 26 
IS  - 5 
UR  - http://genome.cshlp.org/content/26/5/624.abstract 
N2  - RNA polymerase III (Pol III) is tightly controlled in response to environmental cues, yet a genomic-scale picture of Pol III regulation and the role played by its repressor MAF1 is lacking. Here, we describe genome-wide studies in human fibroblasts that reveal a dynamic and gene-specific adaptation of Pol III recruitment to extracellular signals in an mTORC1-dependent manner. Repression of Pol III recruitment and transcription are tightly linked to MAF1, which selectively localizes at Pol III loci, even under serum-replete conditions, and increasingly targets transcribing Pol III in response to serum starvation. Combining Pol III binding profiles with EU-labeling and high-throughput sequencing of newly synthesized small RNAs, we show that Pol III occupancy closely reflects ongoing transcription. Our results exclude the long-term, unproductive arrest of Pol III on the DNA as a major regulatory mechanism and identify previously uncharacterized, differential coordination in Pol III binding and transcription under different growth conditions. 
ER  -