@article{Orioli01052016,
author = {Orioli, Andrea and Praz, Viviane and Lhôte, Philippe and Hernandez, Nouria}, 
title = {Human MAF1 targets and represses active RNA polymerase III genes by preventing recruitment rather than inducing long-term transcriptional arrest},
volume = {26}, 
number = {5}, 
pages = {624-635}, 
year = {2016}, 
doi = {10.1101/gr.201400.115}, 
abstract ={RNA polymerase III (Pol III) is tightly controlled in response to environmental cues, yet a genomic-scale picture of Pol III regulation and the role played by its repressor MAF1 is lacking. Here, we describe genome-wide studies in human fibroblasts that reveal a dynamic and gene-specific adaptation of Pol III recruitment to extracellular signals in an mTORC1-dependent manner. Repression of Pol III recruitment and transcription are tightly linked to MAF1, which selectively localizes at Pol III loci, even under serum-replete conditions, and increasingly targets transcribing Pol III in response to serum starvation. Combining Pol III binding profiles with EU-labeling and high-throughput sequencing of newly synthesized small RNAs, we show that Pol III occupancy closely reflects ongoing transcription. Our results exclude the long-term, unproductive arrest of Pol III on the DNA as a major regulatory mechanism and identify previously uncharacterized, differential coordination in Pol III binding and transcription under different growth conditions.}, 
URL = {http://genome.cshlp.org/content/26/5/624.abstract}, 
eprint = {http://genome.cshlp.org/content/26/5/624.full.pdf+html}, 
journal = {Genome Research} 
}