RT Journal
A1 Pinto, Yishay
A1 Gabay, Orshay
A1 Arbiza, Leonardo
A1 Sams, Aaron J.
A1 Keinan, Alon
A1 Levanon, Erez Y.
T1 Clustered mutations in hominid genome evolution are consistent with APOBEC3G enzymatic activity
JF Genome Research 
JO Genome Research 
YR 2016 
FD May 01 
VO 26 
IS 5 
SP 579 
OP 587 
DO 10.1101/gr.199240.115 
UL http://genome.cshlp.org/content/26/5/579.abstract 
AB The gradual accumulation of mutations by any of a number of mutational processes is a major driving force of divergence and evolution. Here, we investigate a potentially novel mutational process that is based on the activity of members of the AID/APOBEC family of deaminases. This gene family has been recently shown to introduce—in multiple types of cancer—enzyme-induced clusters of co-occurring somatic mutations caused by cytosine deamination. Going beyond somatic mutations, we hypothesized that APOBEC3—following its rapid expansion in primates—can introduce unique germline mutation clusters that can play a role in primate evolution. In this study, we tested this hypothesis by performing a comprehensive comparative genomic screen for APOBEC3-induced mutagenesis patterns across different hominids. We detected thousands of mutation clusters introduced along primate evolution which exhibit features that strongly fit the known patterns of APOBEC3G mutagenesis. These results suggest that APOBEC3G-induced mutations have contributed to the evolution of all genomes we studied. This is the first indication of site-directed, enzyme-induced genome evolution, which played a role in the evolution of both modern and archaic humans. This novel mutational mechanism exhibits several unique features, such as its higher tendency to mutate transcribed regions and regulatory elements and its ability to generate clusters of concurrent point mutations that all occur in a single generation. Our discovery demonstrates the exaptation of an anti-viral mechanism as a new source of genomic variation in hominids with a strong potential for functional consequences.