TY  - JOUR
A1  - Marzi, Matteo J.
A1  - Ghini, Francesco
A1  - Cerruti, Benedetta
A1  - de Pretis, Stefano
A1  - Bonetti, Paola
A1  - Giacomelli, Chiara
A1  - Gorski, Marcin M.
A1  - Kress, Theresia
A1  - Pelizzola, Mattia
A1  - Muller, Heiko
A1  - Amati, Bruno
A1  - Nicassio, Francesco
T1  - Degradation dynamics of microRNAs revealed by a novel pulse-chase approach
Y1  - 2016/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 554 
EP  - 565 
DO  - 10.1101/gr.198788.115 
VL  - 26 
IS  - 4 
UR  - http://genome.cshlp.org/content/26/4/554.abstract 
N2  - The regulation of miRNAs is critical to the definition of cell identity and behavior in normal physiology and disease. To date, the dynamics of miRNA degradation and the mechanisms involved in remain largely obscure, in particular, in higher organisms. Here, we developed a pulse-chase approach based on metabolic RNA labeling to calculate miRNA decay rates at genome-wide scale in mammalian cells. Our analysis revealed heterogeneous miRNA half-lives, with many species behaving as stable molecules (T1/2 > 24 h), while others, including passenger miRNAs and a number (25/129) of guide miRNAs, are quickly turned over (T1/2 = 4–14 h). Decay rates were coupled with other features, including genomic organization, transcription rates, structural heterogeneity (isomiRs), and target abundance, measured through quantitative experimental approaches. This comprehensive analysis highlighted functional mechanisms that mediate miRNA degradation, as well as the importance of decay dynamics in the regulation of the miRNA pool under both steady-state conditions and during cell transitions. 
ER  -