@article{Valli01042016,
author = {Valli, Adrian A. and Santos, Bruno A.C.M. and Hnatova, Silvia and Bassett, Andrew R. and Molnar, Attila and Chung, Betty Y. and Baulcombe, David C.}, 
title = {Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs},
volume = {26}, 
number = {4}, 
pages = {519-529}, 
year = {2016}, 
doi = {10.1101/gr.199703.115}, 
abstract ={We describe here a forward genetic screen to investigate the biogenesis, mode of action, and biological function of miRNA-mediated RNA silencing in the model algal species, Chlamydomonas reinhardtii. Among the mutants from this screen, there were three at Dicer-like 3 that failed to produce both miRNAs and siRNAs and others affecting diverse post-biogenesis stages of miRNA-mediated silencing. The DCL3-dependent siRNAs fell into several classes including transposon- and repeat-derived siRNAs as in higher plants. The DCL3-dependent miRNAs differ from those of higher plants, however, in that many of them are derived from mRNAs or from the introns of pre-mRNAs. Transcriptome analysis of the wild-type and dcl3 mutant strains revealed a further difference from higher plants in that the sRNAs are rarely negative switches of mRNA accumulation. The few transcripts that were more abundant in dcl3 mutant strains than in wild-type cells were not due to sRNA-targeted RNA degradation but to direct DCL3 cleavage of miRNA and siRNA precursor structures embedded in the untranslated (and translated) regions of the mRNAs. Our analysis reveals that the miRNA-mediated RNA silencing in C. reinhardtii differs from that of higher plants and informs about the evolution and function of this pathway in eukaryotes.}, 
URL = {http://genome.cshlp.org/content/26/4/519.abstract}, 
eprint = {http://genome.cshlp.org/content/26/4/519.full.pdf+html}, 
journal = {Genome Research} 
}