TY  - JOUR
A1  - Gesson, Kevin
A1  - Rescheneder, Philipp
A1  - Skoruppa, Michael P.
A1  - von Haeseler, Arndt
A1  - Dechat, Thomas
A1  - Foisner, Roland
T1  - A-type lamins bind both hetero- and euchromatin, the latter being regulated by lamina-associated polypeptide 2 alpha
Y1  - 2016/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 462 
EP  - 473 
DO  - 10.1101/gr.196220.115 
VL  - 26 
IS  - 4 
UR  - http://genome.cshlp.org/content/26/4/462.abstract 
N2  - Lamins are components of the peripheral nuclear lamina and interact with heterochromatic genomic regions, termed lamina-associated domains (LADs). In contrast to lamin B1 being primarily present at the nuclear periphery, lamin A/C also localizes throughout the nucleus, where it associates with the chromatin-binding protein lamina-associated polypeptide (LAP) 2 alpha. Here, we show that lamin A/C also interacts with euchromatin, as determined by chromatin immunoprecipitation of euchromatin- and heterochromatin-enriched samples. By way of contrast, lamin B1 was only found associated with heterochromatin. Euchromatic regions occupied by lamin A/C overlap with those bound by LAP2alpha, and lack of LAP2alpha in LAP2alpha-deficient cells shifts binding of lamin A/C toward more heterochromatic regions. These alterations in lamin A/C-chromatin interactions correlate with changes in epigenetic histone marks in euchromatin but do not significantly affect gene expression. Loss of lamin A/C in heterochromatic regions in LAP2alpha-deficient cells, however, correlated with increased gene expression. Our data show a novel role of nucleoplasmic lamin A/C and LAP2alpha in regulating euchromatin. 
ER  -