@article{Gesson01042016,
author = {Gesson, Kevin and Rescheneder, Philipp and Skoruppa, Michael P. and von Haeseler, Arndt and Dechat, Thomas and Foisner, Roland}, 
title = {A-type lamins bind both hetero- and euchromatin, the latter being regulated by lamina-associated polypeptide 2 alpha},
volume = {26}, 
number = {4}, 
pages = {462-473}, 
year = {2016}, 
doi = {10.1101/gr.196220.115}, 
abstract ={Lamins are components of the peripheral nuclear lamina and interact with heterochromatic genomic regions, termed lamina-associated domains (LADs). In contrast to lamin B1 being primarily present at the nuclear periphery, lamin A/C also localizes throughout the nucleus, where it associates with the chromatin-binding protein lamina-associated polypeptide (LAP) 2 alpha. Here, we show that lamin A/C also interacts with euchromatin, as determined by chromatin immunoprecipitation of euchromatin- and heterochromatin-enriched samples. By way of contrast, lamin B1 was only found associated with heterochromatin. Euchromatic regions occupied by lamin A/C overlap with those bound by LAP2alpha, and lack of LAP2alpha in LAP2alpha-deficient cells shifts binding of lamin A/C toward more heterochromatic regions. These alterations in lamin A/C-chromatin interactions correlate with changes in epigenetic histone marks in euchromatin but do not significantly affect gene expression. Loss of lamin A/C in heterochromatic regions in LAP2alpha-deficient cells, however, correlated with increased gene expression. Our data show a novel role of nucleoplasmic lamin A/C and LAP2alpha in regulating euchromatin.}, 
URL = {http://genome.cshlp.org/content/26/4/462.abstract}, 
eprint = {http://genome.cshlp.org/content/26/4/462.full.pdf+html}, 
journal = {Genome Research} 
}