TY  - JOUR
A1  - Jühling, Frank
A1  - Kretzmer, Helene
A1  - Bernhart, Stephan H.
A1  - Otto, Christian
A1  - Stadler, Peter F.
A1  - Hoffmann, Steve
T1  - metilene: fast and sensitive calling of differentially methylated regions from bisulfite sequencing data
Y1  - 2016/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 256 
EP  - 262 
DO  - 10.1101/gr.196394.115 
VL  - 26 
IS  - 2 
UR  - http://genome.cshlp.org/content/26/2/256.abstract 
N2  - The detection of differentially methylated regions (DMRs) is a necessary prerequisite for characterizing different epigenetic states. We present a novel program, metilene, to identify DMRs within whole-genome and targeted data with unrivaled specificity and sensitivity. A binary segmentation algorithm combined with a two-dimensional statistical test allows the detection of DMRs in large methylation experiments with multiple groups of samples in minutes rather than days using off-the-shelf hardware. metilene outperforms other state-of-the-art tools for low coverage data and can estimate missing data. Hence, metilene is a versatile tool to study the effect of epigenetic modifications in differentiation/development, tumorigenesis, and systems biology on a global, genome-wide level. Whether in the framework of international consortia with dozens of samples per group, or even without biological replicates, it produces highly significant and reliable results. 
ER  -