RT Journal
A1 Wang, Su
A1 Zang, Chongzhi
A1 Xiao, Tengfei
A1 Fan, Jingyu
A1 Mei, Shenglin
A1 Qin, Qian
A1 Wu, Qiu
A1 Li, Xujuan
A1 Xu, Kexin
A1 He, Housheng Hansen
A1 Brown, Myles
A1 Meyer, Clifford A.
A1 Liu, X. Shirley
T1 Modeling cis-regulation with a compendium of genome-wide histone H3K27ac profiles
JF Genome Research 
JO Genome Research 
YR 2016 
FD October 01 
VO 26 
IS 10 
SP 1417 
OP 1429 
DO 10.1101/gr.201574.115 
UL http://genome.cshlp.org/content/26/10/1417.abstract 
AB Model-based analysis of regulation of gene expression (MARGE) is a framework for interpreting the relationship between the H3K27ac chromatin environment and differentially expressed gene sets. The framework has three main functions: MARGE-potential, MARGE-express, and MARGE-cistrome. MARGE-potential defines a regulatory potential (RP) for each gene as the sum of H3K27ac ChIP-seq signals weighted by a function of genomic distance from the transcription start site. The MARGE framework includes a compendium of RPs derived from 365 human and 267 mouse H3K27ac ChIP-seq data sets. Relative RPs, scaled using this compendium, are superior to superenhancers in predicting BET (bromodomain and extraterminal domain) -inhibitor repressed genes. MARGE-express, which uses logistic regression to retrieve relevant H3K27ac profiles from the compendium to accurately model a query set of differentially expressed genes, was tested on 671 diverse gene sets from MSigDB. MARGE-cistrome adopts a novel semisupervised learning approach to identify cis-regulatory elements regulating a gene set. MARGE-cistrome exploits information from H3K27ac signal at DNase I hypersensitive sites identified from published human and mouse DNase-seq data. We tested the framework on newly generated RNA-seq and H3K27ac ChIP-seq profiles upon siRNA silencing of multiple transcriptional and epigenetic regulators in a prostate cancer cell line, LNCaP-abl. MARGE-cistrome can predict the binding sites of silenced transcription factors without matched H3K27ac ChIP-seq data. Even when the matching H3K27ac ChIP-seq profiles are available, MARGE leverages public H3K27ac profiles to enhance these data. This study demonstrates the advantage of integrating a large compendium of historical epigenetic data for genomic studies of transcriptional regulation.