TY  - JOUR
A1  - McSweeney, K. Melodi
A1  - Gussow, Ayal B.
A1  - Bradrick, Shelton S.
A1  - Dugger, Sarah A.
A1  - Gelfman, Sahar
A1  - Wang, Quanli
A1  - Petrovski, Slavé
A1  - Frankel, Wayne N.
A1  - Boland, Michael J.
A1  - Goldstein, David B.
T1  - Inhibition of microRNA 128 promotes excitability of cultured cortical neuronal networks
Y1  - 2016/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1411 
EP  - 1416 
DO  - 10.1101/gr.199828.115 
VL  - 26 
IS  - 10 
UR  - http://genome.cshlp.org/content/26/10/1411.abstract 
N2  - Cultured neuronal networks monitored with microelectrode arrays (MEAs) have been used widely to evaluate pharmaceutical compounds for potential neurotoxic effects. A newer application of MEAs has been in the development of in vitro models of neurological disease. Here, we directly evaluated the utility of MEAs to recapitulate in vivo phenotypes of mature microRNA-128 (miR-128) deficiency, which causes fatal seizures in mice. We show that inhibition of miR-128 results in significantly increased neuronal activity in cultured neuronal networks derived from primary mouse cortical neurons. These results support the utility of MEAs in developing in vitro models of neuroexcitability disorders, such as epilepsy, and further suggest that MEAs provide an effective tool for the rapid identification of microRNAs that promote seizures when dysregulated. 
ER  -