RT Journal
A1 Navin, Nicholas E.
T1 The first five years of single-cell cancer genomics and beyond
JF Genome Research 
JO Genome Research 
YR 2015 
FD October 01 
VO 25 
IS 10 
SP 1499 
OP 1507 
DO 10.1101/gr.191098.115 
UL http://genome.cshlp.org/content/25/10/1499.abstract 
AB Single-cell sequencing (SCS) is a powerful new tool for investigating evolution and diversity in cancer and understanding the role of rare cells in tumor progression. These methods have begun to unravel key questions in cancer biology that have been difficult to address with bulk tumor measurements. Over the past five years, there has been extraordinary progress in technological developments and research applications, but these efforts represent only the tip of the iceberg. In the coming years, SCS will greatly improve our understanding of invasion, metastasis, and therapy resistance during cancer progression. These tools will also have direct translational applications in the clinic, in areas such as early detection, noninvasive monitoring, and guiding targeted therapy. In this perspective, I discuss the progress that has been made and the myriad of unexplored applications that still lie ahead in cancer research and medicine.