@article{Gansauge01092014,
author = {Gansauge, Marie-Theres and Meyer, Matthias}, 
title = {Selective enrichment of damaged DNA molecules for ancient genome sequencing},
volume = {24}, 
number = {9}, 
pages = {1543-1549}, 
year = {2014}, 
doi = {10.1101/gr.174201.114}, 
abstract ={Contamination by present-day human and microbial DNA is one of the major hindrances for large-scale genomic studies using ancient biological material. We describe a new molecular method, U selection, which exploits one of the most distinctive features of ancient DNA—the presence of deoxyuracils—for selective enrichment of endogenous DNA against a complex background of contamination during DNA library preparation. By applying the method to Neanderthal DNA extracts that are heavily contaminated with present-day human DNA, we show that the fraction of useful sequence information increases ∼10-fold and that the resulting sequences are more efficiently depleted of human contamination than when using purely computational approaches. Furthermore, we show that U selection can lead to a four- to fivefold increase in the proportion of endogenous DNA sequences relative to those of microbial contaminants in some samples. U selection may thus help to lower the costs for ancient genome sequencing of nonhuman samples also.}, 
URL = {http://genome.cshlp.org/content/24/9/1543.abstract}, 
eprint = {http://genome.cshlp.org/content/24/9/1543.full.pdf+html}, 
journal = {Genome Research} 
}