TY  - JOUR
A1  - Shi, Weibing
A1  - Moon, Christina D.
A1  - Leahy, Sinead C.
A1  - Kang, Dongwan
A1  - Froula, Jeff
A1  - Kittelmann, Sandra
A1  - Fan, Christina
A1  - Deutsch, Samuel
A1  - Gagic, Dragana
A1  - Seedorf, Henning
A1  - Kelly, William J.
A1  - Atua, Renee
A1  - Sang, Carrie
A1  - Soni, Priya
A1  - Li, Dong
A1  - Pinares-Patiño, Cesar S.
A1  - McEwan, John C.
A1  - Janssen, Peter H.
A1  - Chen, Feng
A1  - Visel, Axel
A1  - Wang, Zhong
A1  - Attwood, Graeme T.
A1  - Rubin, Edward M.
T1  - Methane yield phenotypes linked to differential gene expression in the sheep rumen microbiome
Y1  - 2014/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1517 
EP  - 1525 
DO  - 10.1101/gr.168245.113 
VL  - 24 
IS  - 9 
UR  - http://genome.cshlp.org/content/24/9/1517.abstract 
N2  - Ruminant livestock represent the single largest anthropogenic source of the potent greenhouse gas methane, which is generated by methanogenic archaea residing in ruminant digestive tracts. While differences between individual animals of the same breed in the amount of methane produced have been observed, the basis for this variation remains to be elucidated. To explore the mechanistic basis of this methane production, we measured methane yields from 22 sheep, which revealed that methane yields are a reproducible, quantitative trait. Deep metagenomic and metatranscriptomic sequencing demonstrated a similar abundance of methanogens and methanogenesis pathway genes in high and low methane emitters. However, transcription of methanogenesis pathway genes was substantially increased in sheep with high methane yields. These results identify a discrete set of rumen methanogens whose methanogenesis pathway transcription profiles correlate with methane yields and provide new targets for CH4 mitigation at the levels of microbiota composition and transcriptional regulation. 
ER  -