RT Journal
A1 Turelli, Priscilla
A1 Castro-Diaz, Nathaly
A1 Marzetta, Flavia
A1 Kapopoulou, Adamandia
A1 Raclot, Charlène
A1 Duc, Julien
A1 Tieng, Vannary
A1 Quenneville, Simon
A1 Trono, Didier
T1 Interplay of TRIM28 and DNA methylation in controlling human endogenous retroelements
JF Genome Research 
JO Genome Research 
YR 2014 
FD August 01 
VO 24 
IS 8 
SP 1260 
OP 1270 
DO 10.1101/gr.172833.114 
UL http://genome.cshlp.org/content/24/8/1260.abstract 
AB Reverse transcription-derived sequences account for at least half of the human genome. Although these retroelements are formidable motors of evolution, they can occasionally cause disease, and accordingly are inactivated during early embryogenesis through epigenetic mechanisms. In the mouse, at least for endogenous retroviruses, important mediators of this process are the tetrapod-specific KRAB-containing zinc finger proteins (KRAB-ZFPs) and their cofactor TRIM28. The present study demonstrates that KRAB/TRIM28-mediated regulation is responsible for controlling a very broad range of human-specific endogenous retroelements (EREs) in human embryonic stem (ES) cells and that it exerts, as a consequence, a marked effect on the transcriptional dynamics of these cells. It further reveals reciprocal dependence between TRIM28 recruitment at specific families of EREs and DNA methylation. It finally points to the importance of persistent TRIM28-mediated control of ERE transcriptional impact beyond their presumed inactivation by DNA methylation.