RT Journal
A1 Orsi, Guillermo A.
A1 Kasinathan, Sivakanthan
A1 Hughes, Kelly T.
A1 Saminadin-Peter, Sarah
A1 Henikoff, Steven
A1 Ahmad, Kami
T1 High-resolution mapping defines the cooperative architecture of Polycomb response elements
JF Genome Research 
JO Genome Research 
YR 2014 
FD May 01 
VO 24 
IS 5 
SP 809 
OP 820 
DO 10.1101/gr.163642.113 
UL http://genome.cshlp.org/content/24/5/809.abstract 
AB Polycomb-mediated chromatin repression modulates gene expression during development in metazoans. Binding of multiple sequence-specific factors at discrete Polycomb response elements (PREs) is thought to recruit repressive complexes that spread across an extended chromatin domain. To dissect the structure of PREs, we applied high-resolution mapping of nonhistone chromatin proteins in native chromatin of Drosophila cells. Analysis of occupied sites reveal interactions between transcription factors that stabilize Polycomb anchoring to DNA, and implicate the general transcription factor ADF1 as a novel PRE component. By comparing two Drosophila cell lines with differential chromatin states, we provide evidence that repression is accomplished by enhanced Polycomb recruitment both to PREs and to target promoters of repressed genes. These results suggest that the stability of multifactor complexes at promoters and regulatory elements is a crucial aspect of developmentally regulated gene expression.