TY  - JOUR
A1  - Symmons, Orsolya
A1  - Uslu, Veli Vural
A1  - Tsujimura, Taro
A1  - Ruf, Sandra
A1  - Nassari, Sonya
A1  - Schwarzer, Wibke
A1  - Ettwiller, Laurence
A1  - Spitz, François
T1  - Functional and topological characteristics of mammalian regulatory domains
Y1  - 2014/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 390 
EP  - 400 
DO  - 10.1101/gr.163519.113 
VL  - 24 
IS  - 3 
UR  - http://genome.cshlp.org/content/24/3/390.abstract 
N2  - Long-range regulatory interactions play an important role in shaping gene-expression programs. However, the genomic features that organize these activities are still poorly characterized. We conducted a large operational analysis to chart the distribution of gene regulatory activities along the mouse genome, using hundreds of insertions of a regulatory sensor. We found that enhancers distribute their activities along broad regions and not in a gene-centric manner, defining large regulatory domains. Remarkably, these domains correlate strongly with the recently described TADs, which partition the genome into distinct self-interacting blocks. Different features, including specific repeats and CTCF-binding sites, correlate with the transition zones separating regulatory domains, and may help to further organize promiscuously distributed regulatory influences within large domains. These findings support a model of genomic organization where TADs confine regulatory activities to specific but large regulatory domains, contributing to the establishment of specific gene expression profiles. 
ER  -