TY  - JOUR
A1  - Sawicka, Anna
A1  - Hartl, Dominik
A1  - Goiser, Malgorzata
A1  - Pusch, Oliver
A1  - Stocsits, Roman R.
A1  - Tamir, Ido M.
A1  - Mechtler, Karl
A1  - Seiser, Christian
T1  - H3S28 phosphorylation is a hallmark of the transcriptional response to cellular stress
Y1  - 2014/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1808 
EP  - 1820 
DO  - 10.1101/gr.176255.114 
VL  - 24 
IS  - 11 
UR  - http://genome.cshlp.org/content/24/11/1808.abstract 
N2  - The selectivity of transcriptional responses to extracellular cues is reflected by the deposition of stimulus-specific chromatin marks. Although histone H3 phosphorylation is a target of numerous signaling pathways, its role in transcriptional regulation remains poorly understood. Here, for the first time, we report a genome-wide analysis of H3S28 phosphorylation in a mammalian system in the context of stress signaling. We found that this mark targets as many as 50% of all stress-induced genes, underlining its importance in signal-induced transcription. By combining ChIP-seq, RNA-seq, and mass spectrometry we identified the factors involved in the biological interpretation of this histone modification. We found that MSK1/2-mediated phosphorylation of H3S28 at stress-responsive promoters contributes to the dissociation of HDAC corepressor complexes and thereby to enhanced local histone acetylation and subsequent transcriptional activation of stress-induced genes. Our data reveal a novel function of the H3S28ph mark in the activation of mammalian genes in response to MAP kinase pathway activation. 
ER  -