TY  - JOUR
A1  - Sung, Young Hoon
A1  - Kim, Jong Min
A1  - Kim, Hyun-Taek
A1  - Lee, Jaehoon
A1  - Jeon, Jisun
A1  - Jin, Young
A1  - Choi, Jung-Hwa
A1  - Ban, Young Ho
A1  - Ha, Sang-Jun
A1  - Kim, Cheol-Hee
A1  - Lee, Han-Woong
A1  - Kim, Jin-Soo
T1  - Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases
Y1  - 2014/01/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 125 
EP  - 131 
DO  - 10.1101/gr.163394.113 
VL  - 24 
IS  - 1 
UR  - http://genome.cshlp.org/content/24/1/125.abstract 
N2  - RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the mouse Prkdc gene that encodes an enzyme critical for DNA double-strand break repair resulted in immunodeficiency both in F0 and F1 mice. We propose that RGEN-mediated mutagenesis in animals will greatly expedite the creation of genetically engineered model organisms, accelerating functional genomic research. 
ER  -