@article{Sung01012014,
author = {Sung, Young Hoon and Kim, Jong Min and Kim, Hyun-Taek and Lee, Jaehoon and Jeon, Jisun and Jin, Young and Choi, Jung-Hwa and Ban, Young Ho and Ha, Sang-Jun and Kim, Cheol-Hee and Lee, Han-Woong and Kim, Jin-Soo}, 
title = {Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases},
volume = {24}, 
number = {1}, 
pages = {125-131}, 
year = {2014}, 
doi = {10.1101/gr.163394.113}, 
abstract ={RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the mouse Prkdc gene that encodes an enzyme critical for DNA double-strand break repair resulted in immunodeficiency both in F0 and F1 mice. We propose that RGEN-mediated mutagenesis in animals will greatly expedite the creation of genetically engineered model organisms, accelerating functional genomic research.}, 
URL = {http://genome.cshlp.org/content/24/1/125.abstract}, 
eprint = {http://genome.cshlp.org/content/24/1/125.full.pdf+html}, 
journal = {Genome Research} 
}