RT Journal
A1 Grimaldi, Yoselin
A1 Ferrari, Paolo
A1 Strubin, Michel
T1 Independent RNA polymerase II preinitiation complex dynamics and nucleosome turnover at promoter sites in vivo
JF Genome Research 
JO Genome Research 
YR 2014 
FD January 01 
VO 24 
IS 1 
SP 117 
OP 124 
DO 10.1101/gr.157792.113 
UL http://genome.cshlp.org/content/24/1/117.abstract 
AB Transcription by all three eukaryotic RNA polymerases involves the assembly of a large preinitiation complex (PIC) at gene promoters. The PIC comprises several general transcription factors (GTFs), including TBP, and the respective RNA polymerase. It has been suggested that some GTFs remain stably bound at active promoters to facilitate multiple transcription events. Here we used two complementary approaches to show that, in G1-arrested yeast cells, TBP exchanges very rapidly even at the most highly active RNA Pol II promoters. A similar situation is observed at RNA Pol III promoters. In contrast, TBP remains stably bound at RNA Pol I promoters. We also provide evidence that, unexpectedly, PIC dynamics are neither the cause nor the consequence of nucleosome exchange at most of the RNA Pol II promoters we analyzed. These results point to a stable reinitiation complex at RNA Pol I promoters and suggest independent PIC and nucleosome turnover at many RNA Pol II promoters.