RT Journal
A1 Varshney, Gaurav K.
A1 Lu, Jing
A1 Gildea, Derek E.
A1 Huang, Haigen
A1 Pei, Wuhong
A1 Yang, Zhongan
A1 Huang, Sunny C.
A1 Schoenfeld, David
A1 Pho, Nam H.
A1 Casero, David
A1 Hirase, Takashi
A1 Mosbrook-Davis, Deborah
A1 Zhang, Suiyuan
A1 Jao, Li-En
A1 Zhang, Bo
A1 Woods, Ian G.
A1 Zimmerman, Steven
A1 Schier, Alexander F.
A1 Wolfsberg, Tyra G.
A1 Pellegrini, Matteo
A1 Burgess, Shawn M.
A1 Lin, Shuo
T1 A large-scale zebrafish gene knockout resource for the genome-wide study of gene function
JF Genome Research 
JO Genome Research 
YR 2013 
FD April 01 
VO 23 
IS 4 
SP 727 
OP 735 
DO 10.1101/gr.151464.112 
UL http://genome.cshlp.org/content/23/4/727.abstract 
AB With the completion of the zebrafish genome sequencing project, it becomes possible to analyze the function of zebrafish genes in a systematic way. The first step in such an analysis is to inactivate each protein-coding gene by targeted or random mutation. Here we describe a streamlined pipeline using proviral insertions coupled with high-throughput sequencing and mapping technologies to widely mutagenize genes in the zebrafish genome. We also report the first 6144 mutagenized and archived F1's predicted to carry up to 3776 mutations in annotated genes. Using in vitro fertilization, we have rescued and characterized ∼0.5% of the predicted mutations, showing mutation efficacy and a variety of phenotypes relevant to both developmental processes and human genetic diseases. Mutagenized fish lines are being made freely available to the public through the Zebrafish International Resource Center. These fish lines establish an important milestone for zebrafish genetics research and should greatly facilitate systematic functional studies of the vertebrate genome.