@article{Wu01022013,
author = {Wu, Jun and Wang, Zhiwen and Shi, Zebin and Zhang, Shu and Ming, Ray and Zhu, Shilin and Khan, M. Awais and Tao, Shutian and Korban, Schuyler S. and Wang, Hao and Chen, Nancy J. and Nishio, Takeshi and Xu, Xun and Cong, Lin and Qi, Kaijie and Huang, Xiaosan and Wang, Yingtao and Zhao, Xiang and Wu, Juyou and Deng, Cao and Gou, Caiyun and Zhou, Weili and Yin, Hao and Qin, Gaihua and Sha, Yuhui and Tao, Ye and Chen, Hui and Yang, Yanan and Song, Yue and Zhan, Dongliang and Wang, Juan and Li, Leiting and Dai, Meisong and Gu, Chao and Wang, Yuezhi and Shi, Daihu and Wang, Xiaowei and Zhang, Huping and Zeng, Liang and Zheng, Danman and Wang, Chunlei and Chen, Maoshan and Wang, Guangbiao and Xie, Lin and Sovero, Valpuri and Sha, Shoufeng and Huang, Wenjiang and Zhang, Shujun and Zhang, Mingyue and Sun, Jiangmei and Xu, Linlin and Li, Yuan and Liu, Xing and Li, Qingsong and Shen, Jiahui and Wang, Junyi and Paull, Robert E. and Bennetzen, Jeffrey L. and Wang, Jun and Zhang, Shaoling}, 
title = {The genome of the pear (Pyrus bretschneideri Rehd.)},
volume = {23}, 
number = {2}, 
pages = {396-408}, 
year = {2013}, 
doi = {10.1101/gr.144311.112}, 
abstract ={The draft genome of the pear (Pyrus bretschneideri) using a combination of BAC-by-BAC and next-generation sequencing is reported. A 512.0-Mb sequence corresponding to 97.1% of the estimated genome size of this highly heterozygous species is assembled with 194× coverage. High-density genetic maps comprising 2005 SNP markers anchored 75.5% of the sequence to all 17 chromosomes. The pear genome encodes 42,812 protein-coding genes, and of these, ∼28.5% encode multiple isoforms. Repetitive sequences of 271.9 Mb in length, accounting for 53.1% of the pear genome, are identified. Simulation of eudicots to the ancestor of Rosaceae has reconstructed nine ancestral chromosomes. Pear and apple diverged from each other ∼5.4–21.5 million years ago, and a recent whole-genome duplication (WGD) event must have occurred 30–45 MYA prior to their divergence, but following divergence from strawberry. When compared with the apple genome sequence, size differences between the apple and pear genomes are confirmed mainly due to the presence of repetitive sequences predominantly contributed by transposable elements (TEs), while genic regions are similar in both species. Genes critical for self-incompatibility, lignified stone cells (a unique feature of pear fruit), sorbitol metabolism, and volatile compounds of fruit have also been identified. Multiple candidate SFB genes appear as tandem repeats in the S-locus region of pear; while lignin synthesis-related gene family expansion and highly expressed gene families of HCT, C3′H, and CCOMT contribute to high accumulation of both G-lignin and S-lignin. Moreover, alpha-linolenic acid metabolism is a key pathway for aroma in pear fruit.}, 
URL = {http://genome.cshlp.org/content/23/2/396.abstract}, 
eprint = {http://genome.cshlp.org/content/23/2/396.full.pdf+html}, 
journal = {Genome Research} 
}