TY  - JOUR
A1  - Seitan, Vlad C.
A1  - Faure, Andre J.
A1  - Zhan, Ye
A1  - McCord, Rachel Patton
A1  - Lajoie, Bryan R.
A1  - Ing-Simmons, Elizabeth
A1  - Lenhard, Boris
A1  - Giorgetti, Luca
A1  - Heard, Edith
A1  - Fisher, Amanda G.
A1  - Flicek, Paul
A1  - Dekker, Job
A1  - Merkenschlager, Matthias
T1  - Cohesin-based chromatin interactions enable regulated gene expression within preexisting architectural compartments
Y1  - 2013/12/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2066 
EP  - 2077 
DO  - 10.1101/gr.161620.113 
VL  - 23 
IS  - 12 
UR  - http://genome.cshlp.org/content/23/12/2066.abstract 
N2  - Chromosome conformation capture approaches have shown that interphase chromatin is partitioned into spatially segregated Mb-sized compartments and sub-Mb-sized topological domains. This compartmentalization is thought to facilitate the matching of genes and regulatory elements, but its precise function and mechanistic basis remain unknown. Cohesin controls chromosome topology to enable DNA repair and chromosome segregation in cycling cells. In addition, cohesin associates with active enhancers and promoters and with CTCF to form long-range interactions important for gene regulation. Although these findings suggest an important role for cohesin in genome organization, this role has not been assessed on a global scale. Unexpectedly, we find that architectural compartments are maintained in noncycling mouse thymocytes after genetic depletion of cohesin in vivo. Cohesin was, however, required for specific long-range interactions within compartments where cohesin-regulated genes reside. Cohesin depletion diminished interactions between cohesin-bound sites, whereas alternative interactions between chromatin features associated with transcriptional activation and repression became more prominent, with corresponding changes in gene expression. Our findings indicate that cohesin-mediated long-range interactions facilitate discrete gene expression states within preexisting chromosomal compartments. 
ER  -