RT Journal
A1 Thompson, Owen
A1 Edgley, Mark
A1 Strasbourger, Pnina
A1 Flibotte, Stephane
A1 Ewing, Brent
A1 Adair, Ryan
A1 Au, Vinci
A1 Chaudhry, Iasha
A1 Fernando, Lisa
A1 Hutter, Harald
A1 Kieffer, Armelle
A1 Lau, Joanne
A1 Lee, Norris
A1 Miller, Angela
A1 Raymant, Greta
A1 Shen, Bin
A1 Shendure, Jay
A1 Taylor, Jon
A1 Turner, Emily H.
A1 Hillier, LaDeana W.
A1 Moerman, Donald G.
A1 Waterston, Robert H.
T1 The million mutation project: A new approach to genetics in Caenorhabditis elegans
JF Genome Research 
JO Genome Research 
YR 2013 
FD October 01 
VO 23 
IS 10 
SP 1749 
OP 1762 
DO 10.1101/gr.157651.113 
UL http://genome.cshlp.org/content/23/10/1749.abstract 
AB We have created a library of 2007 mutagenized Caenorhabditis elegans strains, each sequenced to a target depth of 15-fold coverage, to provide the research community with mutant alleles for each of the worm's more than 20,000 genes. The library contains over 800,000 unique single nucleotide variants (SNVs) with an average of eight nonsynonymous changes per gene and more than 16,000 insertion/deletion (indel) and copy number changes, providing an unprecedented genetic resource for this multicellular organism. To supplement this collection, we also sequenced 40 wild isolates, identifying more than 630,000 unique SNVs and 220,000 indels. Comparison of the two sets demonstrates that the mutant collection has a much richer array of both nonsense and missense mutations than the wild isolate set. We also find a wide range of rDNA and telomere repeat copy number in both sets. Scanning the mutant collection for molecular phenotypes reveals a nonsense suppressor as well as strains with higher levels of indels that harbor mutations in DNA repair genes and strains with abundant males associated with him mutations. All the strains are available through the Caenorhabditis Genetics Center and all the sequence changes have been deposited in WormBase and are available through an interactive website.