TY  - JOUR
A1  - Thompson, Owen
A1  - Edgley, Mark
A1  - Strasbourger, Pnina
A1  - Flibotte, Stephane
A1  - Ewing, Brent
A1  - Adair, Ryan
A1  - Au, Vinci
A1  - Chaudhry, Iasha
A1  - Fernando, Lisa
A1  - Hutter, Harald
A1  - Kieffer, Armelle
A1  - Lau, Joanne
A1  - Lee, Norris
A1  - Miller, Angela
A1  - Raymant, Greta
A1  - Shen, Bin
A1  - Shendure, Jay
A1  - Taylor, Jon
A1  - Turner, Emily H.
A1  - Hillier, LaDeana W.
A1  - Moerman, Donald G.
A1  - Waterston, Robert H.
T1  - The million mutation project: A new approach to genetics in Caenorhabditis elegans
Y1  - 2013/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1749 
EP  - 1762 
DO  - 10.1101/gr.157651.113 
VL  - 23 
IS  - 10 
UR  - http://genome.cshlp.org/content/23/10/1749.abstract 
N2  - We have created a library of 2007 mutagenized Caenorhabditis elegans strains, each sequenced to a target depth of 15-fold coverage, to provide the research community with mutant alleles for each of the worm's more than 20,000 genes. The library contains over 800,000 unique single nucleotide variants (SNVs) with an average of eight nonsynonymous changes per gene and more than 16,000 insertion/deletion (indel) and copy number changes, providing an unprecedented genetic resource for this multicellular organism. To supplement this collection, we also sequenced 40 wild isolates, identifying more than 630,000 unique SNVs and 220,000 indels. Comparison of the two sets demonstrates that the mutant collection has a much richer array of both nonsense and missense mutations than the wild isolate set. We also find a wide range of rDNA and telomere repeat copy number in both sets. Scanning the mutant collection for molecular phenotypes reveals a nonsense suppressor as well as strains with higher levels of indels that harbor mutations in DNA repair genes and strains with abundant males associated with him mutations. All the strains are available through the Caenorhabditis Genetics Center and all the sequence changes have been deposited in WormBase and are available through an interactive website. 
ER  -