RT Journal
A1 Smallwood, Andrea
A1 Hon, Gary C.
A1 Jin, Fulai
A1 Henry, Ryan E.
A1 Espinosa, Joaquín M.
A1 Ren, Bing
T1 CBX3 regulates efficient RNA processing genome-wide
JF Genome Research 
JO Genome Research 
YR 2012 
FD August 01 
VO 22 
IS 8 
SP 1426 
OP 1436 
DO 10.1101/gr.124818.111 
UL http://genome.cshlp.org/content/22/8/1426.abstract 
AB CBX5, CBX1, and CBX3 (HP1α, β, and γ, respectively) play an evolutionarily conserved role in the formation and maintenance of heterochromatin. In addition, CBX5, CBX1, and CBX3 may also participate in transcriptional regulation of genes. Recently, CBX3 binding to the bodies of a subset of genes has been observed in human and murine cells. However, the generality of this phenomenon and the role CBX3 may play in this context are unknown. Genome-wide localization analysis reveals CBX3 binding at genic regions, which strongly correlates with gene activity across multiple cell types. Depletion of CBX3 resulted in down-regulation of a subset of target genes. Loss of CBX3 binding leads to a more dramatic accumulation of unspliced nascent transcripts. In addition, we observed defective recruitment of splicing factors, including SNRNP70, to CBX3 target genes. Collectively, our data suggest a role for CBX3 in aiding in efficient cotranscriptional RNA processing.