@article{Jovanovic01072012,
author = {Jovanovic, Marko and Reiter, Lukas and Clark, Alejandra and Weiss, Manuel and Picotti, Paola and Rehrauer, Hubert and Frei, Andreas and Neukomm, Lukas J. and Kaufman, Ethan and Wollscheid, Bernd and Simard, Martin J. and Miska, Eric A. and Aebersold, Ruedi and Gerber, André P. and Hengartner, Michael O.}, 
title = {RIP-chip-SRM—a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans},
volume = {22}, 
number = {7}, 
pages = {1360-1371}, 
year = {2012}, 
doi = {10.1101/gr.133330.111}, 
abstract ={MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression. As miRNAs are involved in a wide range of biological processes and diseases, much effort has been invested in identifying their mRNA targets. Here, we present a novel combinatorial approach, RIP-chip-SRM (RNA-binding protein immunopurification + microarray + targeted protein quantification via selected reaction monitoring), to identify de novo high-confidence miRNA targets in the nematode Caenorhabditis elegans. We used differential RIP-chip analysis of miRNA-induced silencing complexes from wild-type and miRNA mutant animals, followed by quantitative targeted proteomics via selected reaction monitoring to identify and validate mRNA targets of the C. elegans bantam homolog miR-58. Comparison of total mRNA and protein abundance changes in mir-58 mutant and wild-type animals indicated that the direct bantam/miR-58 targets identified here are mainly regulated at the level of protein abundance, not mRNA stability.}, 
URL = {http://genome.cshlp.org/content/22/7/1360.abstract}, 
eprint = {http://genome.cshlp.org/content/22/7/1360.full.pdf+html}, 
journal = {Genome Research} 
}