TY  - JOUR
A1  - Kim, Eunji
A1  - Kim, Sojung
A1  - Kim, Duk Hyoung
A1  - Choi, Beom-Soon
A1  - Choi, Ik-Young
A1  - Kim, Jin-Soo
T1  - Precision genome engineering with programmable DNA-nicking enzymes
Y1  - 2012/07/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1327 
EP  - 1333 
DO  - 10.1101/gr.138792.112 
VL  - 22 
IS  - 7 
UR  - http://genome.cshlp.org/content/22/7/1327.abstract 
N2  - Zinc finger nucleases (ZFNs) are powerful tools of genome engineering but are limited by their inevitable reliance on error-prone nonhomologous end-joining (NHEJ) repair of DNA double-strand breaks (DSBs), which gives rise to randomly generated, unwanted small insertions or deletions (indels) at both on-target and off-target sites. Here, we present programmable DNA-nicking enzymes (nickases) that produce single-strand breaks (SSBs) or nicks, instead of DSBs, which are repaired by error-free homologous recombination (HR) rather than mutagenic NHEJ. Unlike their corresponding nucleases, zinc finger nickases allow site-specific genome modifications only at the on-target site, without the induction of unwanted indels. We propose that programmable nickases will be of broad utility in research, medicine, and biotechnology, enabling precision genome engineering in any cell or organism. 
ER  -