TY  - JOUR
A1  - Murray, John Isaac
A1  - Boyle, Thomas J.
A1  - Preston, Elicia
A1  - Vafeados, Dionne
A1  - Mericle, Barbara
A1  - Weisdepp, Peter
A1  - Zhao, Zhongying
A1  - Bao, Zhirong
A1  - Boeck, Max
A1  - Waterston, Robert H.
T1  - Multidimensional regulation of gene expression in the C. elegans embryo
Y1  - 2012/07/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1282 
EP  - 1294 
DO  - 10.1101/gr.131920.111 
VL  - 22 
IS  - 7 
UR  - http://genome.cshlp.org/content/22/7/1282.abstract 
N2  - How cells adopt different expression patterns is a fundamental question of developmental biology. We quantitatively measured reporter expression of 127 genes, primarily transcription factors, in every cell and with high temporal resolution in C. elegans embryos. Embryonic cells are highly distinct in their gene expression; expression of the 127 genes studied here can distinguish nearly all pairs of cells, even between cells of the same tissue type. We observed recurrent lineage-regulated expression patterns for many genes in diverse contexts. These patterns are regulated in part by the TCF-LEF transcription factor POP-1. Other genes' reporters exhibited patterns correlated with tissue, position, and left–right asymmetry. Sequential patterns both within tissues and series of sublineages suggest regulatory pathways. Expression patterns often differ between embryonic and larval stages for the same genes, emphasizing the importance of profiling expression in different stages. This work greatly expands the number of genes in each of these categories and provides the first large-scale, digitally based, cellular resolution compendium of gene expression dynamics in live animals. The resulting data sets will be a useful resource for future research. 
ER  -