RT Journal
A1 Adey, Andrew
A1 Shendure, Jay
T1 Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing
JF Genome Research 
JO Genome Research 
YR 2012 
FD June 01 
VO 22 
IS 6 
SP 1139 
OP 1143 
DO 10.1101/gr.136242.111 
UL http://genome.cshlp.org/content/22/6/1139.abstract 
AB We have adapted transposase-based in vitro shotgun library construction (“tagmentation”) for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6× high-quality coverage of each strand.