RT Journal
A1 Kostic, Aleksandar D.
A1 Gevers, Dirk
A1 Pedamallu, Chandra Sekhar
A1 Michaud, Monia
A1 Duke, Fujiko
A1 Earl, Ashlee M.
A1 Ojesina, Akinyemi I.
A1 Jung, Joonil
A1 Bass, Adam J.
A1 Tabernero, Josep
A1 Baselga, José
A1 Liu, Chen
A1 Shivdasani, Ramesh A.
A1 Ogino, Shuji
A1 Birren, Bruce W.
A1 Huttenhower, Curtis
A1 Garrett, Wendy S.
A1 Meyerson, Matthew
T1 Genomic analysis identifies association of Fusobacterium with colorectal carcinoma
JF Genome Research 
JO Genome Research 
YR 2012 
FD February 01 
VO 22 
IS 2 
SP 292 
OP 298 
DO 10.1101/gr.126573.111 
UL http://genome.cshlp.org/content/22/2/292.abstract 
AB The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.