RT Journal
A1 Ribeiro, Filipe J.
A1 Przybylski, Dariusz
A1 Yin, Shuangye
A1 Sharpe, Ted
A1 Gnerre, Sante
A1 Abouelleil, Amr
A1 Berlin, Aaron M.
A1 Montmayeur, Anna
A1 Shea, Terrance P.
A1 Walker, Bruce J.
A1 Young, Sarah K.
A1 Russ, Carsten
A1 Nusbaum, Chad
A1 MacCallum, Iain
A1 Jaffe, David B.
T1 Finished bacterial genomes from shotgun sequence data
JF Genome Research 
JO Genome Research 
YR 2012 
FD November 01 
VO 22 
IS 11 
SP 2270 
OP 2277 
DO 10.1101/gr.141515.112 
UL http://genome.cshlp.org/content/22/11/2270.abstract 
AB Exceptionally accurate genome reference sequences have proven to be of great value to microbial researchers. Thus, to date, about 1800 bacterial genome assemblies have been “finished” at great expense with the aid of manual laboratory and computational processes that typically iterate over a period of months or even years. By applying a new laboratory design and new assembly algorithm to 16 samples, we demonstrate that assemblies exceeding finished quality can be obtained from whole-genome shotgun data and automated computation. Cost and time requirements are thus dramatically reduced.