TY - JOUR A1 - Ribeiro, Filipe J. A1 - Przybylski, Dariusz A1 - Yin, Shuangye A1 - Sharpe, Ted A1 - Gnerre, Sante A1 - Abouelleil, Amr A1 - Berlin, Aaron M. A1 - Montmayeur, Anna A1 - Shea, Terrance P. A1 - Walker, Bruce J. A1 - Young, Sarah K. A1 - Russ, Carsten A1 - Nusbaum, Chad A1 - MacCallum, Iain A1 - Jaffe, David B. T1 - Finished bacterial genomes from shotgun sequence data Y1 - 2012/11/01 JF - Genome Research JO - Genome Research SP - 2270 EP - 2277 DO - 10.1101/gr.141515.112 VL - 22 IS - 11 UR - http://genome.cshlp.org/content/22/11/2270.abstract N2 - Exceptionally accurate genome reference sequences have proven to be of great value to microbial researchers. Thus, to date, about 1800 bacterial genome assemblies have been “finished” at great expense with the aid of manual laboratory and computational processes that typically iterate over a period of months or even years. By applying a new laboratory design and new assembly algorithm to 16 samples, we demonstrate that assemblies exceeding finished quality can be obtained from whole-genome shotgun data and automated computation. Cost and time requirements are thus dramatically reduced. ER -