RT Journal
A1 Rao, Nagesha A.S.
A1 McCalman, Melysia T.
A1 Moulos, Panagiotis
A1 Francoijs, Kees-Jan
A1 Chatziioannou, Aristotelis
A1 Kolisis, Fragiskos N.
A1 Alexis, Michael N.
A1 Mitsiou, Dimitra J.
A1 Stunnenberg, Hendrik G.
T1 Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes
JF Genome Research 
JO Genome Research 
YR 2011 
FD September 01 
VO 21 
IS 9 
SP 1404 
OP 1416 
DO 10.1101/gr.118042.110 
UL http://genome.cshlp.org/content/21/9/1404.abstract 
AB Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.