RT Journal
A1 Arnold, Christopher P.
A1 Tan, Ruoying
A1 Zhou, Baiyu
A1 Yue, Si-Biao
A1 Schaffert, Steven
A1 Biggs, Joseph R.
A1 Doyonnas, Regis
A1 Lo, Miao-Chia
A1 Perry, John M.
A1 Renault, Valérie M.
A1 Sacco, Alessandra
A1 Somervaille, Tim
A1 Viatour, Patrick
A1 Brunet, Anne
A1 Cleary, Michael L.
A1 Li, Linheng
A1 Sage, Julien
A1 Zhang, Dong-Er
A1 Blau, Helen M.
A1 Chen, Caifu
A1 Chen, Chang-Zheng
T1 MicroRNA programs in normal and aberrant stem and progenitor cells
JF Genome Research 
JO Genome Research 
YR 2011 
FD May 01 
VO 21 
IS 5 
SP 798 
OP 810 
DO 10.1101/gr.111385.110 
UL http://genome.cshlp.org/content/21/5/798.abstract 
AB Emerging evidence suggests that microRNAs (miRNAs), an abundant class of ∼22-nucleotide small regulatory RNAs, play key roles in controlling the post-transcriptional genetic programs in stem and progenitor cells. Here we systematically examined miRNA expression profiles in various adult tissue-specific stem cells and their differentiated counterparts. These analyses revealed miRNA programs that are common or unique to blood, muscle, and neural stem cell populations and miRNA signatures that mark the transitions from self-renewing and quiescent stem cells to proliferative and differentiating progenitor cells. Moreover, we identified a stem/progenitor transition miRNA (SPT-miRNA) signature that predicts the effects of genetic perturbations, such as loss of PTEN and the Rb family, AML1-ETO9a expression, and MLL-AF10 transformation, on self-renewal and proliferation potentials of mutant stem/progenitor cells. We showed that some of the SPT-miRNAs control the self-renewal of embryonic stem cells and the reconstitution potential of hematopoietic stem cells (HSCs). Finally, we demonstrated that SPT-miRNAs coordinately regulate genes that are known to play roles in controlling HSC self-renewal, such as Hoxb6 and Hoxa4. Together, these analyses reveal the miRNA programs that may control key processes in normal and aberrant stem and progenitor cells, setting the foundations for dissecting post-transcriptional regulatory networks in stem cells.