RT Journal
A1 Nguyen, Di Kim
A1 Yang, Fan
A1 Kaul, Rajinder
A1 Alkan, Can
A1 Antonellis, Anthony
A1 Friery, Karen F.
A1 Zhu, Baoli
A1 de Jong, Pieter J.
A1 Disteche, Christine M.
T1 Clcn4-2 genomic structure differs between the X locus in Mus spretus and the autosomal locus in Mus musculus: AT motif enrichment on the X
JF Genome Research 
JO Genome Research 
YR 2011 
FD March 01 
VO 21 
IS 3 
SP 402 
OP 409 
DO 10.1101/gr.108563.110 
UL http://genome.cshlp.org/content/21/3/402.abstract 
AB In Mus spretus, the chloride channel 4 gene Clcn4-2 is X-linked and dosage compensated by X up-regulation and X inactivation, while in the closely related mouse species Mus musculus, Clcn4-2 has been translocated to chromosome 7. We sequenced Clcn4-2 in M. spretus and identified the breakpoints of the evolutionary translocation in the Mus lineage. Genetic and epigenetic differences were observed between the 5′ends of the autosomal and X-linked loci. Remarkably, Clcn4-2 introns have been truncated on chromosome 7 in M. musculus as compared with the X-linked loci from seven other eutherian mammals. Intron sequences specifically preserved in the X-linked loci were significantly enriched in AT-rich oligomers. Genome-wide analyses showed an overall enrichment in AT motifs unique to the eutherian X (except for genes that escape X inactivation), suggesting a role for these motifs in regulation of the X chromosome.