RT Journal
A1 Ramani, Arun K.
A1 Calarco, John A.
A1 Pan, Qun
A1 Mavandadi, Sepand
A1 Wang, Ying
A1 Nelson, Andrew C.
A1 Lee, Leo J.
A1 Morris, Quaid
A1 Blencowe, Benjamin J.
A1 Zhen, Mei
A1 Fraser, Andrew G.
T1 Genome-wide analysis of alternative splicing in Caenorhabditis elegans
JF Genome Research 
JO Genome Research 
YR 2011 
FD February 01 
VO 21 
IS 2 
SP 342 
OP 348 
DO 10.1101/gr.114645.110 
UL http://genome.cshlp.org/content/21/2/342.abstract 
AB Alternative splicing (AS) plays a crucial role in the diversification of gene function and regulation. Consequently, the systematic identification and characterization of temporally regulated splice variants is of critical importance to understanding animal development. We have used high-throughput RNA sequencing and microarray profiling to analyze AS in C. elegans across various stages of development. This analysis identified thousands of novel splicing events, including hundreds of developmentally regulated AS events. To make these data easily accessible and informative, we constructed the C. elegans Splice Browser, a web resource in which researchers can mine AS events of interest and retrieve information about their relative levels and regulation across development. The data presented in this study, along with the Splice Browser, provide the most comprehensive set of annotated splice variants in C. elegans to date, and are therefore expected to facilitate focused, high resolution in vivo functional assays of AS function.