TY  - JOUR
A1  - Daines, Bryce
A1  - Wang, Hui
A1  - Wang, Liguo
A1  - Li, Yumei
A1  - Han, Yi
A1  - Emmert, David
A1  - Gelbart, William
A1  - Wang, Xia
A1  - Li, Wei
A1  - Gibbs, Richard
A1  - Chen, Rui
T1  - The Drosophila melanogaster transcriptome by paired-end RNA sequencing
Y1  - 2011/02/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 315 
EP  - 324 
DO  - 10.1101/gr.107854.110 
VL  - 21 
IS  - 2 
UR  - http://genome.cshlp.org/content/21/2/315.abstract 
N2  - RNA-seq was used to generate an extensive map of the Drosophila melanogaster transcriptome by broad sampling of 10 developmental stages. In total, 142.2 million uniquely mapped 64–100-bp paired-end reads were generated on the Illumina GA II yielding 356× sequencing coverage. More than 95% of FlyBase genes and 90% of splicing junctions were observed. Modifications to 30% of FlyBase gene models were made by extension of untranslated regions, inclusion of novel exons, and identification of novel splicing events. A total of 319 novel transcripts were identified, representing a 2% increase over the current annotation. Alternate splicing was observed in 31% of D. melanogaster genes, a 38% increase over previous estimations, but significantly less than that observed in higher organisms. Much of this splicing is subtle such as tandem alternate splice sites. 
ER  -