TY  - JOUR
A1  - Komori, H. Kiyomi
A1  - LaMere, Sarah A.
A1  - Torkamani, Ali
A1  - Hart, G. Traver
A1  - Kotsopoulos, Steve
A1  - Warner, Jason
A1  - Samuels, Michael L.
A1  - Olson, Jeff
A1  - Head, Steven R.
A1  - Ordoukhanian, Phillip
A1  - Lee, Pauline L.
A1  - Link, Darren R.
A1  - Salomon, Daniel R.
T1  - Application of microdroplet PCR for large-scale targeted bisulfite sequencing
Y1  - 2011/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1738 
EP  - 1745 
DO  - 10.1101/gr.116863.110 
VL  - 21 
IS  - 10 
UR  - http://genome.cshlp.org/content/21/10/1738.abstract 
N2  - Cytosine methylation of DNA CpG dinucleotides in gene promoters is an epigenetic modification that regulates gene transcription. While many methods exist to interrogate methylation states, few current methods offer large-scale, targeted, single CpG resolution. We report an approach combining bisulfite treatment followed by microdroplet PCR with next-generation sequencing to assay the methylation state of 50 genes in the regions 1 kb upstream of and downstream from their transcription start sites. This method yielded 96% coverage of the targeted CpGs and demonstrated high correlation between CpG island (CGI) DNA methylation and transcriptional regulation. The method was scaled to interrogate the methylation status of 77,674 CpGs in the promoter regions of 2100 genes in primary CD4 T cells. The 2100 gene library yielded 97% coverage of all targeted CpGs and 99% of the target amplicons. 
ER  -