RT Journal
A1 Wu, Weisheng
A1 Cheng, Yong
A1 Keller, Cheryl A.
A1 Ernst, Jason
A1 Kumar, Swathi Ashok
A1 Mishra, Tejaswini
A1 Morrissey, Christapher
A1 Dorman, Christine M.
A1 Chen, Kuan-Bei
A1 Drautz, Daniela
A1 Giardine, Belinda
A1 Shibata, Yoichiro
A1 Song, Lingyun
A1 Pimkin, Max
A1 Crawford, Gregory E.
A1 Furey, Terrence S.
A1 Kellis, Manolis
A1 Miller, Webb
A1 Taylor, James
A1 Schuster, Stephan C.
A1 Zhang, Yu
A1 Chiaromonte, Francesca
A1 Blobel, Gerd A.
A1 Weiss, Mitchell J.
A1 Hardison, Ross C.
T1 Dynamics of the epigenetic landscape during erythroid differentiation after GATA1 restoration
JF Genome Research 
JO Genome Research 
YR 2011 
FD October 01 
VO 21 
IS 10 
SP 1659 
OP 1671 
DO 10.1101/gr.125088.111 
UL http://genome.cshlp.org/content/21/10/1659.abstract 
AB Interplays among lineage-specific nuclear proteins, chromatin modifying enzymes, and the basal transcription machinery govern cellular differentiation, but their dynamics of action and coordination with transcriptional control are not fully understood. Alterations in chromatin structure appear to establish a permissive state for gene activation at some loci, but they play an integral role in activation at other loci. To determine the predominant roles of chromatin states and factor occupancy in directing gene regulation during differentiation, we mapped chromatin accessibility, histone modifications, and nuclear factor occupancy genome-wide during mouse erythroid differentiation dependent on the master regulatory transcription factor GATA1. Notably, despite extensive changes in gene expression, the chromatin state profiles (proportions of a gene in a chromatin state dominated by activating or repressive histone modifications) and accessibility remain largely unchanged during GATA1-induced erythroid differentiation. In contrast, gene induction and repression are strongly associated with changes in patterns of transcription factor occupancy. Our results indicate that during erythroid differentiation, the broad features of chromatin states are established at the stage of lineage commitment, largely independently of GATA1. These determine permissiveness for expression, with subsequent induction or repression mediated by distinctive combinations of transcription factors.