RT Journal
A1 Hu, Gangqing
A1 Schones, Dustin E.
A1 Cui, Kairong
A1 Ybarra, River
A1 Northrup, Daniel
A1 Tang, Qingsong
A1 Gattinoni, Luca
A1 Restifo, Nicholas P.
A1 Huang, Suming
A1 Zhao, Keji
T1 Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1
JF Genome Research 
JO Genome Research 
YR 2011 
FD October 01 
VO 21 
IS 10 
SP 1650 
OP 1658 
DO 10.1101/gr.121145.111 
UL http://genome.cshlp.org/content/21/10/1650.abstract 
AB Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.