RT Journal
A1 Minot, Samuel
A1 Sinha, Rohini
A1 Chen, Jun
A1 Li, Hongzhe
A1 Keilbaugh, Sue A.
A1 Wu, Gary D.
A1 Lewis, James D.
A1 Bushman, Frederic D.
T1 The human gut virome: Inter-individual variation and dynamic response to diet
JF Genome Research 
JO Genome Research 
YR 2011 
FD October 01 
VO 21 
IS 10 
SP 1616 
OP 1625 
DO 10.1101/gr.122705.111 
UL http://genome.cshlp.org/content/21/10/1616.abstract 
AB Immense populations of viruses are present in the human gut and other body sites. Understanding the role of these populations (the human “virome”) in health and disease requires a much deeper understanding of their composition and dynamics in the face of environmental perturbation. Here, we investigate viromes from human subjects on a controlled feeding regimen. Longitudinal fecal samples were analyzed by metagenomic sequencing of DNA from virus-like particles (VLP) and total microbial communities. Assembly of 336 Mb of VLP sequence yielded 7175 contigs, many identifiable as complete or partial bacteriophage genomes. Contigs were rich in viral functions required in lytic and lysogenic growth, as well as unexpected functions such as viral CRISPR arrays and genes for antibiotic resistance. The largest source of variance among virome samples was interpersonal variation. Parallel deep-sequencing analysis of bacterial populations showed covaration of the virome with the larger microbiome. The dietary intervention was associated with a change in the virome community to a new state, in which individuals on the same diet converged. Thus these data provide an overview of the composition of the human gut virome and associate virome structure with diet.