RT Journal
A1 Mondal, Tanmoy
A1 Rasmussen, Markus
A1 Pandey, Gaurav Kumar
A1 Isaksson, Anders
A1 Kanduri, Chandrasekhar
T1 Characterization of the RNA content of chromatin
JF Genome Research 
JO Genome Research 
YR 2010 
FD July 01 
VO 20 
IS 7 
SP 899 
OP 907 
DO 10.1101/gr.103473.109 
UL http://genome.cshlp.org/content/20/7/899.abstract 
AB Noncoding RNA (ncRNA) constitutes a significant portion of the mammalian transcriptome. Emerging evidence suggests that it regulates gene expression in cis or trans by modulating the chromatin structure. To uncover the functional role of ncRNA in chromatin organization, we deep sequenced chromatin-associated RNAs (CARs) from human fibroblast (HF) cells. This resulted in the identification of 141 intronic regions and 74 intergenic regions harboring CARs. The intronic and intergenic CARs show significant conservation across 44 species of placental mammals. Functional characterization of one of the intergenic CARs, Intergenic10, revealed that it regulates gene expression of neighboring genes through modulating the chromatin structure in cis. Our data suggest that ncRNA is an integral component of chromatin and that it may regulate various biological functions through fine-tuning of the chromatin architecture.